(1) The RECIPIENT shall obtain a prior agreement from Dr. Hiroshi Sasaki (sasaki@cdb.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0502K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Cell. 2008 15(1):23-36.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
条件を付加する。<br>(1) 分与希望者は事前に 佐々木 洋 (sasaki@cdb.riken.jp) に連絡をとり、分与に関して許可を得ること。(2) 当該マウスを用いる論文発表に際してはAccession No. CDB0502K、そのWEB サイトhttp://www2.clst.riken.jp/arg/mutant%20mice%20list.htmlとともに当該マウスを記載すること。(3) 当該マウスを用いる論文発表に際しては、その由来として第一報論文 Dev Cell. 2008 15(1):23-36. を引用すること。（4）発表論文を寄託者（mutant.bdr@riken.jp）に連絡すること。
FLP/frt system
<a href='https://brc.riken.jp/mus/pcr03519'>Genotyping protocol -PCR-</a>
Cthrc1(lacZ) (Acc.No. CDB0502K)
Cthrc1(lacZ) (Acc.No. CDB0502K)
C (3-6 months)
RBRC03519
Developed by Hiroshi Sasaki, RIKEN Center for Developmental Biology. A lacZ and PGK-neo cassette was transferred into E14TG2a ES cells to replace the exon 1 of Cthrc1 gene.
C（3〜6か月）
B6D2;129P2-Cthrc1<tm1Hssk>/Rbrc
true
Neo : E. coli, lacZ : E. coli, lox71 and loxP : P1 Phage, frt : yeast FRT (flipase recombination target) sites, PGK promoter and PGK 3' end : mouse, rabbit beta-globin 3' end : Rabbit
RIKEN CDB
RIKEN CDB
Cre/loxP system
E14TG2a [129P2/OlaHsd]
Cthrc1(lacZ) (Acc.No. CDB0502K)
Fluorescent Proteins/lacZ System
Cthrc1 gene knockout mice. Cthrc1 is a Wnt signaling component that selectively activates the PCP pathway.
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>